ABSTRACT
Objective To evaluate the link between phenotypic traits, antimicrobial resistance, and biofilm-producing capacity of urinary isolates of Citrobacter freundii (C. Freundii). Methods Both pan-antibiotic-susceptible and -resistant C. freundii isolates (n = 120) obtained from laboratory-confirmed urinary tract infections were analyzed for their link between antimicrobial resistance, phenotypic characteristics, and biofilm production. Results Of the total C. freundii isolates (n = 120), 30% (37/120) of them formed large colonies. Among the total large colonies produced (n = 37), they were present in 21.62%, 10.81%, 13.5%, 16.2%, 21.62%, and 16.21% in the control group, CIP-group, FOS-group, COT-group, Gent-group, and ESBL groups, respectively. Compared to the pan-susceptible isolates, the occurrence of large-sized-colony-forming strains was relatively reduced in most of the drug-resistant groups. The overall distribution of mucoid colonies produced (n = 54) includes 9.25%, 18.51%, 16.66%, 18.51%, 20.3%, and 16.66% in the control group, CIP-group, FOS-group, COT-group, Gent-group, and ESBL groups, respectively. Of the total isolates that produced biofilm (n = 51), 11.76% of isolates showed biofilm formation in the control group. Alternatively, the rate was found to be 15.68%, 11.76%, 25.49%, 19.6%, and 15.68% in the CIP-group, FOS-group, SXT-group, Gen-group, and ESBL-groups, respectively. Conclusion This study correlates the association between phenotypic characteristics, antimicrobial resistance, and biofilm production, the three main characteristics of C. Freundii.
ABSTRACT
Background: Tuberculosis (TB) is one of the major cause of morbidity and mortality worldwide, affecting one third of world’s population and the incidence is much higher in South-East Asia (India and China together account for nearly 40% of the global TB cases) Objectives: The current study was designed to evaluate the efficacy of relative centrifugal forces (RCFs) and various centrifugations times (CTs) on recovery of Mycobacterium tuberculosis (MTB) from sputum specimens pre-inoculated with MTB. Materials & Methods: For this study, direct acid fast bacilli (AFB) smear negative sputum specimens were randomly collected, which were further subjected to sterilization ( by autoclaving at 1210 C for 15 min ) and then seeded with MTB. Afterwards, these sputum specimens were subjected to centrifugation (in a cold centrifuge) at various RCFs and CTs. After centrifugation, supernatants and sediments were inoculated on LJ media and incubated at 370 C with daily monitoring for 8 weeks. Results: In the current study, as the RCFs (6000xg) and CTs ( 25 min and 20 min) were increased, the growth detection time exponentially decreased (9 and 11 days) and culture grades (4+) were increased when cultured from the sediments. However, the recovery rates of MTB from the supernatant fluids were constantly decreased as RCFs and CTs increased. Conclusion: The current study concluded that a higher RCF of 6000xg and CTs of 20 and 25 min could effectively detect the tubercle bacilli from the sputum specimens more efficiently than other lower RCFs and CTs.